Local television footage showed a line of dozens of vehicles stranded on the side of a key coastal highway south of Lima and hundreds of protesters placing stones on roads in the Andean regions of Puno, Cusco and Arequipa.
Peru announced a nationwide state of emergency on Wednesday, granting police special powers and limiting freedoms including the right to assembly, after Castillo's removal fueled a week of protests that have left at least eight dead.
Castillo, a leftist elected in 2021, was ousted on Dec. 7 in an impeachment vote and promptly arrested after illegally trying to dissolve Congress. His former vice president, Dina Boluarte, was sworn into office after his removal.
Prosecutors are seeking 18 months of pretrial detention for Castillo, who has been charged with rebellion and conspiracy.
Peru's supreme court has until Friday to decide.
Castillo has the denied accusations and on Wednesday in a message on Twitter asked the Inter-American Commission on Human Rights to "intercede" on behalf of his rights.
Peru's General Confederation of Workers, one of the country's largest unions, has called for a "day of fighting", amid protests demanding immediate elections, the closure of Congress, a constituent assembly and the resignation of Boluarte.
(Reporting by Marco Aquino; Writing by Valentine Hilaire; Editing by Anthony Esposito and Alistair Bell)
